Monoclonal antibody 3F8 recognises the neural cell adhesion molecule (NCAM) in addition to the ganglioside GD2
1989
Monoclonal Antibody 3F8 and Its Binding to NCAM
publication
Evidence: moderate
Author Information
Author(s): K. Patel, R.J. Rossell, L.F. Pemberton, N.K. Cheung, F.S. Walsh, S.E. Moore, T. Sugimoto, J.T. Kemshead
Hypothesis
Does monoclonal antibody 3F8 bind to the neural cell adhesion molecule (NCAM) in addition to the ganglioside GD2?
Conclusion
Monoclonal antibody 3F8 reacts with NCAM as well as GD2, indicating a need to re-evaluate its binding specificity for clinical use.
Supporting Evidence
- 3F8 was originally observed to have a binding profile similar to two other monoclonal antibodies that bind to NCAM.
- Cross-reactivity of 3F8 with NCAM was confirmed by blocking studies.
- 3F8 binds to human NCAM from 3T3 fibroblasts transfected with NCAM cDNA constructs.
Takeaway
This study found that a special antibody called 3F8 can stick to two different things in the body, which might change how we use it in medicine.
Methodology
The study involved immunoprecipitation, Western blot analysis, and cross-blocking studies to assess the binding of antibody 3F8 to NCAM.
Limitations
The study does not provide specific limitations.
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