Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production
2006
Caveolin-1 and Murine Leukaemia Virus Interaction
publication
Evidence: moderate
Author Information
Author(s): Yu Zheng, Christiane Beer, Mario Koester, Manfred Wirth
Primary Institution: German Research Centre for Biotechnology
Hypothesis
Does caveolin-1 interact with the Gag precursor of murine leukaemia virus and modulate virus production?
Conclusion
Caveolin-1 interacts with MLV Gag, co-localizes with Gag at the plasma membrane, and affects the production of infectious virus.
Supporting Evidence
- Caveolin-1 co-localizes with Gag at the plasma membrane of NIH 3T3 cells.
- Caveolin-1 is incorporated into MLV released from NIH 3T3 cells.
- Overexpression of a caveolin-1 binding domain reduces infectious retrovirus production.
Takeaway
Caveolin-1 helps a virus called MLV to make more copies of itself by sticking to a part of the virus's building blocks.
Methodology
The study used co-immunoprecipitation and confocal microscopy to analyze the interaction between caveolin-1 and MLV Gag.
Digital Object Identifier (DOI)
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