Coenzyme Q10 Reduces Ethanol-Induced Apoptosis in Corneal Fibroblasts
Author Information
Author(s): Chen Chun-Chen, Liou Shiow-Wen, Chen Chi-Chih, Chen Wen-Chung, Hu Fung-Rong, Wang I-Jong, Lin Shing-Jong
Primary Institution: Department of Ophthalmology, Taipei City Hospital Renai Branch, Taipei, Taiwan
Hypothesis
CoQ10 pretreatment increases cell viability and reduces cell apoptosis in corneas exposed to ethanol during refractive surgery.
Conclusion
CoQ10 can inhibit mitochondrial depolarization, caspase activation, and cell apoptosis in corneal fibroblasts after ethanol exposure.
Supporting Evidence
- Ethanol treatment significantly decreased the viability of corneal fibroblasts characterized by a higher percentage of apoptotic cells.
- CoQ10 pretreatment significantly reduced mitochondrial depolarization and ROS production.
- The inhibition of cell apoptosis by CoQ10 was significant at 8 and 12 hours after ethanol exposure.
Takeaway
This study shows that a substance called CoQ10 can help protect eye cells from dying when they are exposed to alcohol.
Methodology
Corneal fibroblasts were pretreated with CoQ10 and then exposed to different concentrations of ethanol, followed by assessments of cell viability and apoptosis.
Limitations
The study was conducted in vitro, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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