Isolation, purification, and full NMR assignments of cyclopamine from Veratrum californicum
2008
Isolation and Purification of Cyclopamine from Veratrum californicum
publication
Evidence: high
Author Information
Author(s): Oatis John E Jr, Brunsfeld Pam, Rushing James W, Moeller Peter D, Bearden Daniel W, Gallien Thomas N, Cooper George IV
Primary Institution: Medical University of South Carolina
Hypothesis
A more efficient method for isolating cyclopamine from Veratrum californicum is needed for research purposes.
Conclusion
This method could meet a very real need within diverse scientific communities by allowing cyclopamine to become more readily available.
Supporting Evidence
- Cyclopamine is a useful reagent for probing the Hedgehog signaling pathway.
- The new method showed that very little cyclopamine remained in the plant material after extraction.
- Quantitative analysis demonstrated that the plant contained 2.34 g of cyclopamine per kg of dried material.
- The isolation method is scalable for in vivo and in vitro research.
Takeaway
The researchers found a better way to get a useful chemical called cyclopamine from a plant, which can help scientists study how it works.
Methodology
The study used Soxhlet extraction with benzene and liquid chromatography to isolate and purify cyclopamine from the plant.
Digital Object Identifier (DOI)
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