Phytoceramide and Sphingoid Bases from Brewer's Yeast Activate PPARs
Author Information
Author(s): Murakami Itsuo, Wakasa Yukari, Yamashita Shinji, Kurihara Toshio, Zama Kota, Kobayashi Naoyuki, Mizutani Yukiko, Mitsutake Susumu, Shigyo Tatsuro, Igarashi Yasuyuki
Primary Institution: Hokkaido University
Hypothesis
Natural sphingolipids with C18 fatty acid and yeast-derived sphingoid bases activate PPARs as PPAR agonists.
Conclusion
Phytoceramide and yeast-derived sphingoid bases activate PPARs, while ceramide and dihydroceramide do not change PPAR activity.
Supporting Evidence
- Phytoceramide significantly increased PPAR activity in NIH/3T3 cells.
- Yeast-derived sphingoid bases also activated PPARs.
- Ceramide and dihydroceramide did not activate PPARs.
- PPARβ was more intensely activated than PPARα and PPARγ by phytoceramide.
Takeaway
This study found that a substance called phytoceramide from yeast can help activate important proteins in our body that manage fat and sugar levels.
Methodology
Lipids were extracted from brewer's yeast, purified, and their effects on PPAR activation were assessed using luciferase reporter assays in NIH/3T3 and HepG2 cells.
Potential Biases
Potential bias may arise from the use of specific cell lines and the controlled laboratory conditions that may not fully represent in vivo conditions.
Limitations
The study does not explore the long-term effects of phytoceramide on PPAR activation or its potential side effects.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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