BiSearch: A Tool for Designing PCR Primers
Author Information
Author(s): Arányi Tamás, Váradi András, Simon István, Tusnády Gábor E
Primary Institution: Institute of Enzymology, BRC, HAS, Budapest, Hungary
Hypothesis
The BiSearch web server improves the efficiency of PCR primer design for bisulfite-treated DNA templates.
Conclusion
BiSearch web server is a useful tool for primer-design for any DNA template, especially for bisulfite-treated genomes.
Supporting Evidence
- BiSearch is approximately hundred times faster than previous versions.
- It can design primers for both native and bisulfite-treated DNA.
- The ePCR tool detects potential mispriming sites effectively.
- BiSearch is the only online tool that predicts PCR amplification products for bisulfite-treated templates.
Takeaway
BiSearch helps scientists create better DNA primers, especially for tricky samples that have been treated to study gene activity.
Methodology
The BiSearch web server uses a primer-design module and an electronic PCR (ePCR) algorithm to optimize primer design and detect mispriming sites.
Limitations
The software may not account for all potential mispriming sites in complex templates.
Digital Object Identifier (DOI)
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