Structural organization of the 19S proteasome lid: Insights from MS of intact complexes
2006
Understanding the Structure of the 19S Proteasome Lid
publication
Evidence: high
Author Information
Author(s): Sharon Michal, Taverner Thomas, Ambroggio Xavier I, Deshaies Raymond J, Robinson Carol V
Primary Institution: Department of Chemistry, University of Cambridge
Hypothesis
What is the structural organization of the 19S proteasome lid?
Conclusion
The study reveals a comprehensive model of the 19S proteasome lid's subunit organization and interactions.
Supporting Evidence
- Eight of the nine subunits of the 19S lid were found to be present stoichiometrically.
- Tandem mass spectrometry revealed the subunit architecture of the lid complex.
- A stable tetrameric subcomplex was identified in solution.
- Cross-linking approaches identified further interaction partners.
- The results allow for the construction of a comprehensive interaction map.
Takeaway
Scientists used special techniques to look at a protein complex and found out how its parts fit together and work.
Methodology
The study employed tandem mass spectrometry and chemical cross-linking to analyze the intact 19S proteasome lid complex.
Limitations
High-resolution structural analyses are challenging due to the dynamic and heterogeneous nature of the complex.
Digital Object Identifier (DOI)
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