IgE Promotes Survival of Lung Mast Cells
Author Information
Author(s): Cruse Glenn, Cockerill Sarah, Bradding Peter
Primary Institution: University of Leicester
Hypothesis
Does IgE alone enhance human lung mast cell survival through the production of IL-6?
Conclusion
IgE in the absence of antigen promotes human lung mast cell survival through the autocrine production of IL-6.
Supporting Evidence
- IgE alone promoted the survival of HLMC in a dose-dependent manner following cytokine withdrawal.
- IgE-induced survival was eliminated with the addition of neutralising anti-IL-6 antibody.
- IL-6 mRNA expression was significantly upregulated in HLMC exposed to IgE.
Takeaway
IgE helps lung mast cells live longer even without allergens by making them produce a substance called IL-6.
Methodology
Human lung mast cells were cultured and survival assays were performed after cytokine withdrawal in the presence or absence of IgE, with IL-6 mRNA expression and protein measured.
Potential Biases
Potential bias due to the use of a single IgE preparation and the variability in mast cell responses.
Limitations
The study was conducted in vitro, and clinical relevance remains to be confirmed.
Participant Demographics
Human lung mast cells were isolated from lung tissue obtained from patients undergoing surgery for bronchial carcinoma.
Statistical Information
P-Value
0.020
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website