Detecting Carbapenemase-Producing Enterobacterales with MacErt Media
Author Information
Author(s): Muacevic Alexander, Adler John R, Sow Ousmane, Cissé Abdoulaye, Ndiaye Issa, Niang Elhadj A, Kane Farma T, Cissé Khoudia, Gueye Adja B, Bawa Aminatou A, Fall Cheikh, Dieye Yakhya, Sambe Bissoume, Seck Abdoulaye
Primary Institution: Institut Pasteur de Dakar
Hypothesis
The study evaluates the performance of MacConkey agar supplemented with ertapenem for detecting carbapenemase-producing Enterobacterales.
Conclusion
MacErt media effectively detects carbapenemase-producing Enterobacterales, with MacErt1 showing 100% sensitivity and MacErt2 demonstrating 93% specificity.
Supporting Evidence
- MacErt1 exhibited 100% sensitivity for all tested CPEs.
- MacErt2 demonstrated 93% specificity and 100% sensitivity for NDM and KPC producers.
- MacErt1 allowed the isolation of 124 CPEs among 150 examined isolates.
- None of the isolates tested positive for blaKPC, blaVIM, or blaIMP.
Takeaway
The study shows that a special type of agar can help find bad bacteria that resist antibiotics, which is important for keeping people safe from infections.
Methodology
The study involved preparing MacConkey agar with ertapenem and testing it with 26 characterized Enterobacterales strains, including CPEs, to assess sensitivity and specificity.
Limitations
The study's robustness is limited by the small sample size and the lack of testing with clinical samples.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website