Study of Duck Enteritis Virus UL55 Protein
Author Information
Author(s): Wu Ying, Cheng Anchun, Wang Mingshu, Zhang Shunchuan, Zhu Dekang, Jia Renyong, Luo Qihui, Chen Zhengli, Chen Xiaoyue
Primary Institution: Sichuan Agricultural University
Hypothesis
The characterization of the duck enteritis virus UL55 protein has not been reported yet.
Conclusion
The duck enteritis virus UL55 protein was successfully expressed in a prokaryotic expression system, and its properties were characterized for the first time.
Supporting Evidence
- The UL55 gene was about 561 bp and was successfully amplified.
- The recombinant protein was overexpressed in E. coli and purified.
- Polyclonal antibodies against the UL55 protein were prepared and characterized.
Takeaway
Scientists studied a protein from a virus that affects ducks, and they found out how to make it in a lab, which can help in understanding the virus better.
Methodology
The UL55 gene was amplified, cloned into a plasmid, expressed in E. coli, and the protein was purified and characterized using various assays.
Digital Object Identifier (DOI)
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