Isolation of human cell hybrids (HeLa x skin fibroblast) expressing a radiation-induced tumour-associated antigen
1989
Isolation of Human Cell Hybrids Expressing Tumor-Associated Antigen
publication
Evidence: moderate
Author Information
Author(s): M.S. Mendonca, J.L. Redpath
Primary Institution: University of California, Irvine
Hypothesis
Can antibody-based panning techniques effectively isolate radiation-induced neoplastic cells from human cell hybrids?
Conclusion
The antibody-based panning technique significantly enriches for p75 expressing cells in irradiated populations.
Supporting Evidence
- The panning technique increased the proportion of positive cells from 0.05% to 10-20%.
- After two panning cycles, a significant number of p75 positive colonies were identified.
- The study demonstrated a high degree of enrichment for p75 expressing cells using the panning technique.
Takeaway
Scientists found a way to use special antibodies to help find cancer cells in a mix of other cells, making it easier to study them.
Methodology
The study used antibody-based panning techniques to isolate p75 positive cells from human cell hybrids after irradiation.
Limitations
The efficiency of the panning technique may be overestimated due to assumptions about cell numbers.
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