Blocking FcR Reduces Scrapie Agent Invasion in Mice
Author Information
Author(s): Uraki Ryuta, Sakudo Akikazu, Michibata Kosuke, Ano Yasuhisa, Kono Jyuri, Yukawa Masayoshi, Onodera Takashi
Primary Institution: University of Tokyo
Hypothesis
The neonatal Fc receptor (nFcR) plays a role in the incorporation of scrapie agents (PrPSc) into the intestine.
Conclusion
The study found that blocking the Fc receptor significantly reduces the incorporation of scrapie agents into the intestinal villi of suckling mice.
Supporting Evidence
- ZAA treatment significantly decreased the incorporation of IgG and PrPSc in the intestinal villi.
- Suckling SCID mice showed lower uptake of PrPSc compared to wild-type mice.
- Immunohistochemical analysis confirmed the presence of IgG and PrPSc in the intestinal villi.
Takeaway
When baby mice were given a blocker, they absorbed less of the bad prion proteins from their food.
Methodology
Immunohistochemistry was used to analyze the incorporation of IgG and PrPSc in the intestinal villi of CD-1 and SCID mice.
Limitations
The study was conducted on a small sample size of mice, which may limit the generalizability of the findings.
Participant Demographics
Fifteen-day-old CD-1 and SCID mice were used in the experiments.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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