Regulation of Toll-like receptors in neonatal mice brains after hypoxia-ischemia
Author Information
Author(s): Linnea Stridh, Peter LP Smith, Andrew S Naylor, Xiaoyang Wang, Carina Mallard
Primary Institution: Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg
Hypothesis
The expression of Toll-like receptors (TLRs) in the neonatal brain and their regulation after hypoxia-ischemia is unknown.
Conclusion
TLR-2 deficiency reduced hypoxic-ischemic injury in neonatal mice, suggesting it plays a role in neonatal brain injury.
Supporting Evidence
- TLR-1 was down-regulated at 30 min and up-regulated at 6 h and 24 h after hypoxia-ischemia.
- TLR-2 was constitutively expressed in astrocytes and a population of neurons in the hypothalamus.
- TLR-2 KO mice showed a decreased infarct volume compared to wild type after hypoxia-ischemia.
Takeaway
This study found that certain receptors in the brain, called TLRs, change after a lack of oxygen in newborn mice, and one of them, TLR-2, helps protect the brain from damage.
Methodology
Neonatal mice were subjected to hypoxia-ischemia, and TLR mRNA expression was analyzed using RT-qPCR and immunohistochemistry.
Participant Demographics
Neonatal C57BL/6 mice, including wild type and TLR knockout strains.
Statistical Information
P-Value
0.0051
Statistical Significance
p = 0.0051
Digital Object Identifier (DOI)
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