The role of the C8 proton of ATP in the regulation of phosphoryl transfer within kinases and synthetases
2011
The Role of C8 Proton of ATP in Kinase and Synthetase Regulation
publication
Evidence: high
Author Information
Author(s): Kenyon Colin P, Steyn Anjo, Roth Robyn L, Steenkamp Paul A, Nkosi Thokozani C, Oldfield Lyndon C
Primary Institution: CSIR, Biosciences, Pretoria, South Africa
Hypothesis
How does the C8-H of ATP regulate phosphoryl transfer in kinases and synthetases?
Conclusion
The C8-H of ATP plays a crucial role in the regulation of enzyme activity in kinases and synthetases.
Supporting Evidence
- The study demonstrated that C8D-ATP is preferred over non-deuterated ATP by kinases and synthetases.
- Kinetic isotope effects (KIE) were observed, indicating that the C8-H of ATP is involved in the reaction mechanism.
- Enzymes showed a significant preference for C8D-ATP at low ATP concentrations.
Takeaway
This study shows that a specific part of ATP helps enzymes work better by making it easier for them to grab onto ATP.
Methodology
Comparative enzyme activity assays were conducted using ATP and ATP deuterated at the C8 position to assess their effects on enzyme activity.
Digital Object Identifier (DOI)
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