Submicroscopic Gametocytes and the Transmission of Antifolate-Resistant Plasmodium falciparum in Western Kenya
2009

Transmission of Drug-Resistant Malaria in Western Kenya

Sample size: 528 publication Evidence: moderate

Author Information

Author(s): Oesterholt Mayke J. A. M., Alifrangis Michael, Sutherland Colin J., Omar Sabah A., Sawa Patrick, Howitt Christina, Gouagna Louis C., Sauerwein Robert W., Bousema Teun

Primary Institution: Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

Hypothesis

Mutant parasites possess a transmission advantage compared to susceptible parasites.

Conclusion

The study found that additional mutations in the dhfr gene were not related to increased malaria transmission.

Supporting Evidence

  • 66.5% of infections expressed mutations at all three dhfr codons prior to treatment.
  • Addition of AS to SP reduced gametocytemia and malaria transmission during follow-up.
  • All infections had at least a double mutation in the dhfr gene.

Takeaway

The study looked at how certain mutations in malaria parasites affect their ability to spread, and found that having more mutations didn't make them spread more easily.

Methodology

Children were treated with different antimalarial drugs, and their blood samples were analyzed for mutations and gametocyte presence.

Limitations

The absence of wild-type infections limited the ability to detect differences in transmission potential.

Participant Demographics

Children aged 6 months to 10 years with P. falciparum mono-infection.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1371/journal.pone.0004364

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