Appearance of Human Plasma Cells Following Differentiation of Human B Cells in NOD/SCID Mouse Spleen
2003

Human B Cells Turn into Plasma Cells in Mice

Sample size: 30 publication 10 minutes Evidence: moderate

Author Information

Author(s): KENTARO KIKUCHI, ZHE-XIONG LIAN, XIAO-SONG HE, AFTAB A. ANSARI, MIYUKI ISHIBASHI, HIROSHI MIYAKAWA, LEONARD D. SHULTZ, SUSUMU IKEHARA, M. ERIC GERSHWIN

Primary Institution: University of California at Davis School of Medicine

Hypothesis

Can human B cells differentiate into plasma cells when transplanted into NOD/SCID mice?

Conclusion

Human B cells can differentiate into functional plasma cells in the murine spleen.

Supporting Evidence

  • Plasma cell numbers increased by 100-fold after transplantation.
  • Human IgM and IgG were detected in serum, indicating functional plasma cells.
  • Distinct CD192 plasma cell population was identified, suggesting maturation changes.

Takeaway

Scientists put human blood cells into mice to see if they could turn into plasma cells, and they found that they did!

Methodology

Human PBMC were transplanted into the spleens of irradiated NOD/SCID mice, and the differentiation of B cells was monitored over several weeks.

Limitations

The study was limited to a specific mouse model and may not fully represent human biology.

Participant Demographics

Human peripheral blood mononuclear cells from healthy controls were used.

Digital Object Identifier (DOI)

10.1080/10446670310001642122

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