The transcription factor ATF3 is upregulated during chondrocyte differentiation and represses cyclin D1 and A gene transcription
2006
ATF3's Role in Chondrocyte Differentiation
publication
Evidence: moderate
Author Information
Author(s): James Claudine G, Woods Anita, Underhill T Michael, Beier Frank
Primary Institution: University of Western Ontario
Hypothesis
The transcription factor ATF3 regulates chondrocyte differentiation by repressing cyclin D1 and A gene transcription.
Conclusion
ATF3 induction facilitates cell cycle exit and terminal differentiation of chondrocytes.
Supporting Evidence
- Atf3 mRNA levels increase during chondrocyte differentiation in vitro and in vivo.
- Overexpression of ATF3 reduces transcription of cyclin D1 and cyclin A.
- ATF3 represses SOX9-dependent transcription and increases RUNX2-dependent transcription.
Takeaway
ATF3 helps chondrocytes stop dividing and start maturing into bone cells.
Methodology
The study used real-time PCR and transient transfection assays to analyze gene expression and transcriptional activity in chondrocytes.
Participant Demographics
Mouse embryonic limb bud cells and CD1 mice were used for the experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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