The role of reactive oxygen intermediates in coccidioidomycosis in mice
Author Information
Author(s): David A Margolis, Suganya Viriyakosol, Joshua Fierer, Theo N Kirkland
Primary Institution: Veterans Affairs San Diego Healthcare System
Hypothesis
What role do reactive oxygen intermediates play in the immune response to coccidioidomycosis?
Conclusion
Oxidative burst may not be required for protective immunity to coccidioidomycosis.
Supporting Evidence
- gp91phox knockout mice showed a more robust inflammatory response to infection.
- Both gp91phox knockout and control mice were protected by immunization with the recombinant Coccidioides protein Ag2/PRA.
- Coccidioides immitis arthroconidia were more resistant to H2O2 treatment than Aspergillus fumigatus spores.
Takeaway
This study looked at how certain immune responses help mice fight off a fungal infection. It found that a specific immune response might not need reactive oxygen to work.
Methodology
The study used gp91phox knockout mice and C57Bl/6 control mice to compare their immune responses to Coccidioides infection through intraperitoneal and intranasal routes.
Limitations
The study primarily used mouse models, which may not fully represent human responses.
Participant Demographics
Mice used were 6-12 weeks old, both male and female.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website