Epigenetics of gene expression in human hepatoma cells: expression profiling the response to inhibition of DNA methylation and histone deacetylation
2006
How DNA Methylation Affects Gene Expression in Liver Cancer Cells
publication
Evidence: high
Author Information
Author(s): Dannenberg Luke O, Edenberg Howard J
Primary Institution: Indiana University School of Medicine
Hypothesis
Many genes in the human hepatoma cell line HepG2 are regulated by DNA methylation and histone deacetylation.
Conclusion
DNA methylation plays a larger role than histone deacetylation in regulating the expression of several genes involved in liver processes in HepG2 cells.
Supporting Evidence
- 5-aza-dC treatment turned on the expression of 211 probe sets that were not detectably expressed in its absence.
- 5-aza-dC had a much larger effect on gene expression in HepG2 cells than did TSA.
- Many genes involved in liver processes were affected by 5-aza-dC treatment.
Takeaway
Scientists found that a special treatment can turn on genes in liver cancer cells that are usually turned off, helping us understand how these genes work.
Methodology
HepG2 cells were treated with 5-aza-dC and/or TSA, and gene expression was measured using Affymetrix microarrays.
Statistical Information
P-Value
0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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