Gene transfer of apoA-I reduces atherosclerosis
Author Information
Author(s): Corinna Lebherz, Julio Sanmiguel, James M Wilson, Daniel J Rader
Primary Institution: University of Pennsylvania School of Medicine
Hypothesis
Does gene transfer of wild-type apoA-I and apoA-I Milano reduce atherosclerosis to a similar extent?
Conclusion
Both wild-type apoA-I and apoA-I Milano significantly reduced atherosclerosis progression to a similar extent.
Supporting Evidence
- AAV8-mediated gene transfer significantly slowed atherosclerosis progression.
- Both forms of apoA-I were equally effective in reducing atherosclerosis.
- Expression of wtApoA-I reduced atherosclerosis by 32% compared to control.
- Expression of ApoA-IM reduced atherosclerosis by 24% compared to control.
Takeaway
Scientists tested two types of proteins that help reduce heart disease in mice, and found they worked equally well.
Methodology
The study used AAV8 vectors to deliver genes for wild-type apoA-I and apoA-I Milano to LDL receptor deficient mice, followed by analysis of atherosclerotic lesions.
Limitations
The study was conducted in a mouse model, which may not fully replicate human responses.
Participant Demographics
LDL receptor deficient mice on a Western diet.
Statistical Information
P-Value
p = 0.02 for wtApoA-I, p = 0.04 for ApoA-IM
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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