Targeting the X Chromosome during Spermatogenesis Induces Y Chromosome Transmission Ratio Distortion and Early Dominant Embryo Lethality in Anopheles gambiae
2008

Genetic Engineering of Mosquitoes to Control Malaria

publication Evidence: high

Author Information

Author(s): Nikolai Windbichler, Philippos Aris Papathanos, Andrea Crisanti

Primary Institution: Imperial College London

Hypothesis

Can targeting the X chromosome in Anopheles gambiae mosquitoes induce a bias towards Y chromosome transmission and cause early embryo lethality?

Conclusion

The study found that genetically modified male mosquitoes caused early embryo lethality in crosses with wild-type females, demonstrating a strong bias towards Y chromosome-carrying spermatozoa.

Supporting Evidence

  • The engineered mosquitoes showed a strong bias towards Y chromosome-carrying spermatozoa.
  • All spermatozoa from the modified males carried the chromosome X cutting enzyme.
  • Crosses between modified males and wild-type females resulted in no viable offspring.

Takeaway

Scientists changed male mosquitoes so that they only pass on the Y chromosome, which makes the babies die early, helping to control malaria spread.

Methodology

The researchers engineered male Anopheles gambiae mosquitoes to express a homing endonuclease during sperm development, targeting the X chromosome.

Limitations

The study primarily focused on laboratory conditions, which may not fully replicate field conditions.

Digital Object Identifier (DOI)

10.1371/journal.pgen.1000291

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