The Tissue Microlocalisation and Cellular Expression of CD163, VEGF, HLA-DR, iNOS, and MRP 8/14 Is Correlated to Clinical Outcome in NSCLC
2011

Protein Expression in Non-Small Cell Lung Cancer and Its Impact on Survival

Sample size: 40 publication Evidence: moderate

Author Information

Author(s): Chandra M. Ohri, Aarti Shikotra, Ruth H. Green, David A. Waller, Peter Bradding

Primary Institution: University of Leicester

Hypothesis

The study investigates the non-macrophage expression of proteins associated with M1 and M2 macrophages in non-small cell lung cancer (NSCLC) and its correlation with clinical outcomes.

Conclusion

The expression of certain proteins in tumor islets and stroma is associated with significant differences in clinical outcomes for NSCLC patients.

Supporting Evidence

  • Increased NM-HLA-DR expression in tumor islets is associated with extended survival.
  • Higher NM-iNOS levels correlate with better survival outcomes.
  • Lower NM-VEGF expression in tumor islets is linked to improved patient survival.

Takeaway

This study found that proteins related to immune cells in lung tumors can help predict how long patients might live after surgery.

Methodology

The study used immunohistochemistry to analyze protein expression in tumor samples from patients with extended and poor survival.

Limitations

The study did not identify the specific cell types expressing the proteins, and the biological roles of these proteins in cancer are not fully understood.

Participant Demographics

{"age":{"extended_survival":69.1,"poor_survival":69.6},"gender":{"male":{"extended_survival":16,"poor_survival":11}},"tumor_stage":{"extended_survival":{"stage_1":13,"stage_2":5,"stage_3a":2,"stage_3b_and_4":0},"poor_survival":{"stage_1":13,"stage_2":3,"stage_3a":2,"stage_3b_and_4":1}}}

Statistical Information

P-Value

{"NM-HLA-DR":"<0.001","NM-iNOS":"0.02","NM-MRP 8/14":"0.02","NM-VEGF":"<0.001","NM-CD163":"0.04"}

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0021874

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