Cellular mRNA Activates Transcription Elongation by Displacing 7SK RNA
2007
Cellular mRNA Activates Transcription Elongation by Displacing 7SK RNA
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Tara M. Young, Michael Tsai, Bin Tian, Michael B. Mathews, Tsafi Pe'ery
Primary Institution: New Jersey Medical School
Hypothesis
Can the 3'UTR of HIC mRNA regulate transcription elongation by displacing 7SK RNA from P-TEFb complexes?
Conclusion
The 3'UTR of HIC mRNA can activate transcription by displacing 7SK RNA from P-TEFb complexes.
Supporting Evidence
- The 3'UTR of HIC mRNA is necessary and sufficient for activating transcription.
- HIC mRNA displaces 7SK RNA from P-TEFb complexes.
- Transfection of HIC 3'UTR resulted in increased transcription levels.
- HEXIM1 overexpression inhibited the activation by HIC 3'UTR.
Takeaway
A part of a gene called the 3'UTR helps another protein called P-TEFb work better by pushing away a blocker called 7SK RNA.
Methodology
The study involved transfection assays in various cell lines to measure the effects of HIC mRNA on transcription.
Limitations
The study primarily focuses on the HIC mRNA and may not generalize to other mRNAs.
Statistical Information
P-Value
p=0.0188
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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