Chemosensitivity Testing in Gastric Cancer Using MTT Assay
Author Information
Author(s): H. Yamaue, H. Tanimura, K. Noguchi, T. Hotta, M. Tani, T. Tsunoda, M. Iwahashi, M. Tamai, S. Iwakura
Primary Institution: Wakayama Medical College
Hypothesis
The study aims to determine the chemosensitivity of fresh human gastric cancer using highly purified tumor cells.
Conclusion
The MTT assay using highly purified tumor cells showed a 60% clinical response rate in patients with gastric cancer.
Supporting Evidence
- The MTT assay had a success rate of 87.9% in testing tumor cells.
- 12 out of 20 patients showed a clinical response based on the MTT assay results.
- Cisplatin and mitomycin were found to be more effective than other tested drugs.
- Purity of tumor cells was greater than 90% after purification.
- Chemosensitivity in differentiated cancer was equivalent to that in non-differentiated cancer.
Takeaway
Doctors tested cancer cells from patients to see which medicines worked best, and they found that 12 out of 20 patients got better.
Methodology
The study involved 58 patients with gastric cancer, using the MTT assay to test the sensitivity of highly purified tumor cells to various chemotherapy drugs.
Potential Biases
Potential bias due to the lack of randomization and the small number of evaluable lesions.
Limitations
The study had a small sample size for evaluating clinical responses and did not include a control group.
Participant Demographics
Patients included 33 with primary gastric lesions and 8 with metastatic lymph nodes, with no prior chemotherapy.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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