Gene Expression Profiling of Alveolar Soft-Part Sarcoma
Author Information
Author(s): Stockwin Luke H, Vistica David T, Kenney Susan, Schrump David S, Butcher Donna O, Raffeld Mark, Shoemaker Robert H
Primary Institution: National Cancer Institute at Frederick
Hypothesis
What are the gene expression patterns in alveolar soft-part sarcoma (ASPS) that could provide insights into its pathobiology?
Conclusion
This study identifies several gene expression targets involved in angiogenesis, metastasis, and myogenic differentiation in ASPS.
Supporting Evidence
- Elevated expression of genes related to angiogenesis, cell proliferation, and metastasis was identified.
- Validation of transcript differentials was performed using quantitative RT-PCR.
- Immunohistochemical analysis confirmed protein expression for several identified genes.
Takeaway
Researchers looked at the genes in a rare cancer called ASPS to find out what makes it grow and spread, which could help in finding better treatments.
Methodology
RNA samples from seven ASPS patients were analyzed using high-density microarrays and validated with quantitative RT-PCR and immunohistochemistry.
Limitations
The study is based on a small sample size of seven patients, which may limit the generalizability of the findings.
Participant Demographics
Patients were predominantly adolescents and young adults aged 15-39 years.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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