Immunophenotyping of Human Solid Tumours Using Monoclonal Antibodies
Author Information
Author(s): Z.G. Kadagidze, N.N. Tupitsyn, A.J. Baryshnikov, Kh.P. Kadyrov, V.M. Blinov, A. Bernard, M. Amiot, L. Boumsell
Primary Institution: All-Union Cancer Research Centre of the AMS USSR
Hypothesis
The study aimed to assess the value of combined application of two types of monoclonal antibodies reacting with solid tumour cells in terms of clinical immunodiagnosis.
Conclusion
The combination of K20 and ICO-10 monoclonal antibodies is helpful for immunophenotyping solid tumours.
Supporting Evidence
- Thy-1 antigen was expressed in 79% of cases of neuroblastoma.
- K20 Mabs specifically reacted with malignant cells of epithelial origin.
- ICO-10 Mabs are helpful for diagnosing neuroblastoma metastases to lymph nodes, adrenal cortex, and bone marrow.
Takeaway
Researchers used special antibodies to help identify different types of tumors in patients, which can help doctors understand and treat cancer better.
Methodology
Tumour tissue samples were obtained from 66 patients during surgical removal of their tumours, and the expression of immunological markers was assayed in cryostat tumour serial sections using indirect immunofluorescence.
Participant Demographics
Patients included various types of solid tumours such as neuroblastoma, sarcomas, and adenocarcinomas.
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