Role of MSH2 in Trypanosoma cruzi's Response to Oxidative Stress
Author Information
Author(s): Campos Priscila C., Silva Viviane G., Furtado Carolina, Machado-Silva Alice, DaRocha Wanderson D., Peloso Eduardo F., Gadelha Fernanda R., Medeiros Marisa H.G., Lana Gustavo de Carvalho, Chen Ying, Barnes Rebecca L., Passos-Silva Danielle Gomes, McCulloch Richard, Machado Carlos Renato, Teixeira Santuza M.R.
Primary Institution: Universidade Federal de Minas Gerais
Hypothesis
The distinct MSH2 isoforms in Trypanosoma cruzi strains influence their response to oxidative stress.
Conclusion
Different strains of Trypanosoma cruzi exhibit varying levels of oxidative stress response due to differences in MSH2 activity.
Supporting Evidence
- T. cruzi II strains accumulate more 8-oxoguanine in the kDNA after oxidative stress than T. cruzi I strains.
- Distinct levels of DNA mismatch repair activity are found among T. cruzi strains.
- MSH2 has a mitochondrial function involved in the response to oxidative stress.
Takeaway
Some types of the Trypanosoma cruzi parasite are better at handling stress than others because of differences in a specific protein called MSH2.
Methodology
The study involved comparing cell survival rates and DNA damage levels in different T. cruzi strains after exposure to genotoxic agents.
Limitations
The inability to generate MSH2 null mutants in T. cruzi limits the understanding of its complete role.
Participant Demographics
Five cloned T. cruzi strains were used, including both T. cruzi I and II lineages.
Statistical Information
P-Value
p≤0.05
Statistical Significance
p≤0.05
Digital Object Identifier (DOI)
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