Karyotype, Sex Determination, and Meiotic Chromosome Behavior in Two Pholcid (Araneomorphae, Pholcidae) Spiders: Implications for Karyotype Evolution
2011

Karyotype and Sex Determination in Two Pholcid Spiders

Sample size: 30 publication Evidence: moderate

Author Information

Author(s): Adriana E. Golding, Leocadia V. Paliulis

Primary Institution: Biology Department, Bucknell University

Hypothesis

What are the karyotypes and sex determination mechanisms of Physocyclus mexicanus and Holocnemus pluchei?

Conclusion

The study found that Physocyclus mexicanus has X0-XX sex determination, while Holocnemus pluchei exhibits XY-XX sex determination, marking the first evidence of this system in pholcids.

Supporting Evidence

  • Less than 2% of pholcid spider species have published karyotypes.
  • Physocyclus mexicanus has a chromosome number of 2n=15 in males and 2n=16 in females.
  • Holocnemus pluchei has a chromosome number of 2n=28 in both males and females.
  • This study provides the first evidence of XY-XX sex determination in pholcids.

Takeaway

Scientists looked at two types of spiders to see how their chromosomes work and found out how they determine if they are boys or girls.

Methodology

Karyotypes were constructed using observations of living and fixed cells, and sex determination mechanisms were verified using micromanipulation.

Digital Object Identifier (DOI)

10.1371/journal.pone.0024748

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