Study on Cell Cycle Proteins in Cervical Samples of HIV/HPV Co-infected Women
Author Information
Author(s): Nicol Alcina F, Pires Andréa Rodrigues Cordovil, de Souza Simone R, Nuovo Gerard J, Grinsztejn Beatriz, Tristão Aparecida, Russomano Fabio B, Velasque Luciane, e Silva José R Lapa, Pirmez Claude
Primary Institution: Instituto Oswaldo Cruz – FIOCRUZ, Rio de Janeiro, Brazil
Hypothesis
Regulatory and cell cycle protein expression might be additionally modified in the cervix of HIV/HPV co-infected women.
Conclusion
Co-infection of HPV and HIV leads to significant increase in the VEGF and p27 expression when compared to HPV+/HIV-negative infection.
Supporting Evidence
- HIV/HPV co-infection was associated with a significant increase in expression of VEGF and p27.
- Decreased Rb expression was evident with increased CIN grade in HPV alone infections.
- Rb expression increased 3-fold with HPV/HIV-1 co-infection compared to HPV infection alone.
- There was a significant increase in Elf-1 expression in HPV+/HIV- women with CIN II/III and tumor.
Takeaway
This study found that women with both HIV and HPV have higher levels of certain proteins that could lead to cancer compared to those with just HPV.
Methodology
The study analyzed the expression of Rb, p27, VEGF, and Elf-1 in 163 cervical samples using immunohistochemistry and Tissue Micro-Array (TMA).
Limitations
The study could not assay high risk-HPV sub-typing in all samples, which prevented comparison between Hr-HPV types with the expression markers in the different CIN grades.
Participant Demographics
The study included 163 women with cervical samples, 92 HPV positive and HIV negative, and 37 HPV/HIV co-infected.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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