Genomic Resource for Wood Formation in Radiata Pine
Author Information
Author(s): Li Xinguo, Wu Harry X, Dillon Shannon K, Southerton Simon G
Primary Institution: CSIRO Plant Industry
Hypothesis
The study aims to sequence a large number of expressed sequence tags (ESTs) from genes involved in wood formation in radiata pine.
Conclusion
The first large scale genomic resource in radiata pine was generated from six developing xylem cDNA libraries, identifying key genes involved in wood formation.
Supporting Evidence
- 6,389 high quality ESTs were collected from 5,952 cDNA clones.
- 3,304 unigenes were generated, including 952 contigs and 2,352 singletons.
- About 97.0% of the ESTs have matches in the UniProt and TIGR databases.
Takeaway
Scientists studied the genes that help trees grow wood by looking at samples from different ages of radiata pine trees. They found important genes that help make wood strong.
Methodology
Six developing xylem cDNA libraries were constructed from earlywood and latewood tissues sampled at juvenile, transition, and mature ages, and sequenced to generate ESTs.
Limitations
The genomic resources for radiata pine are still limited compared to other tree species, and only a small number of ESTs were previously available.
Participant Demographics
Samples were taken from juvenile (7 yrs), transition (11 yrs), and mature (30 yrs) radiata pine trees.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website