Metabolic Changes in Growth Hormone Receptor Mutant Mice
Author Information
Author(s): Schirra Horst Joachim, Anderson Cameron G., Wilson William J., Kerr Linda, Craik David J., Waters Michael J., Lichanska Agnieszka M.
Primary Institution: Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia
Hypothesis
What are the metabolic consequences of altered growth hormone signaling in mutant mice?
Conclusion
The study found significant metabolic changes in growth hormone receptor mutant mice, including obesity and altered metabolite levels.
Supporting Evidence
- Mutant mice exhibited altered liver metabolism, obesity, and insulin resistance.
- NMR metabonomics identified significant changes in lipid and choline metabolism.
- Decreased urinary levels of taurine, citrate, and 2-oxoglutarate were observed in mutant mice.
- Increased levels of trimethylamine, creatine, and creatinine were found in mutant mice.
- Microarray analysis correlated changes in metabolite concentration with gene expression.
- Metabolic profiles shifted from wild-type to that of the 391 mutant as the 569 mice became obese.
- Taurine was identified as a key metabolite involved in enhancing β-oxidation.
Takeaway
Scientists studied mice with changes in their growth hormone receptor and found that these changes made them gain weight and affected their metabolism.
Methodology
The study used microarray analysis of liver tissue and NMR metabonomics of urine and liver tissue to analyze metabolic changes.
Limitations
The study did not analyze urine from GHR−/− mice due to difficulties in obtaining samples.
Participant Demographics
The study involved male mice aged from 2 to 12 months.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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