Thrombospondin-1 and Sepsis Mortality
Author Information
Author(s): Sara McMaken, Matthew C. Exline, Payal Mehta, Melissa Piper, Yijie Wang, Sara N. Fischer, Christie A. Newland, Carrie A. Schrader, Shannon R. Balser, Anasuya Sarkar, Christopher P. Baran, Clay B. Marsh, Charles H. Cook, Gary S. Phillips, Naeem A. Ali
Primary Institution: The Ohio State University
Hypothesis
Does thrombospondin-1 influence survival in murine severe sepsis through effects on local wound healing or bacterial clearance?
Conclusion
TSP-1 deficiency was protective in murine models of peritoneal sepsis, leading to improved survival and bacterial clearance.
Supporting Evidence
- TSP-1−/− mice had a significantly lower mortality rate after cecal ligation and puncture compared to WT mice.
- TSP-1−/− mice showed improved bacterial clearance in peritoneal sepsis models.
- Phagocytic capacity was increased in TSP-1−/− macrophages compared to WT.
Takeaway
Mice without thrombospondin-1 are better at fighting infections and survive sepsis better than those with it.
Methodology
The study used TSP-1 null and wild type mice in cecal ligation puncture and E. coli injection models to assess survival and bacterial clearance.
Potential Biases
The study did not include a transgenic model to confirm if over-expression of TSP-1 would yield similar results.
Limitations
The study only measured select wound healing markers and did not define the mechanisms of increased bacterial clearance.
Participant Demographics
Male C57BL/6J mice aged 30-60 days were used.
Statistical Information
P-Value
p<0.028
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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