Impact of ret/PTC 1 Rearrangement on Gene Regulation in Papillary Thyroid Carcinoma
Author Information
Author(s): Cahill Susanne, Smyth Paul, Finn Stephen P, Denning Karen, Flavin Richard, O'Regan Esther M, Li Jinghuan, Potratz Astrid, Guenther Simone M, Henfrey Richard, O'Leary John J, Sheils Orla
Primary Institution: Dept. of Histopathology, University of Dublin, Trinity College, Dublin, Ireland
Hypothesis
What is the effect of ret/PTC 1 on transcription and post-transcriptional regulation in papillary thyroid carcinoma?
Conclusion
The study identified a distinct set of differentially expressed genes and miRNAs associated with ret/PTC 1 rearrangement in papillary thyroid carcinoma.
Supporting Evidence
- 21 miRNAs showed significant overexpression in PTC cell lines with ret/PTC 1.
- 14 miRNAs showed underexpression in these cell lines compared to normal thyroid.
- 47 genes were identified as upregulated in ret/PTC 1 cell lines.
- 29 genes were found to be downregulated in ret/PTC 1 cell lines.
Takeaway
This study looked at how a specific gene change affects other genes in thyroid cancer, finding many that are turned on or off because of it.
Methodology
The study used DNA microarray and TaqMan® microRNA assays to analyze gene and miRNA expression in thyroid cell lines.
Limitations
The study is based on in vitro models, which may not fully represent in vivo conditions.
Statistical Information
P-Value
<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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