Down-regulation of Fas-L in glioma cells by ribozyme reduces cell apoptosis, tumour-infiltrating cells, and liver damage but accelerates tumour formation in nude mice
2001
Fas-L Down-Regulation in Glioma Cells and Its Effects
publication
Author Information
Author(s): Chio C-C, Wang Y-S, Chen Y-L, Lin S-J, Yang B-C
Primary Institution: Department of Neurosurgery, Chi-Mei Foundation Hospital
Hypothesis
Down-regulation of Fas-L in glioma cells will affect tumor growth and immune response.
Conclusion
Down-regulating Fas-L in glioma cells improves their viability but accelerates tumor formation.
Supporting Evidence
- Fas-L down-regulation led to enhanced growth rates in glioma cells.
- Fas-Lribozyme-carrying cells showed less spontaneous apoptosis compared to controls.
- In nude mice, tumors from Fas-Lribozyme-carrying cells were larger with fewer immune cells.
- Reduced liver damage was observed in mice with Fas-L down-regulated tumors.
Takeaway
Scientists found that reducing a certain protein in brain tumor cells helps the cells survive but makes the tumors grow faster.
Methodology
The study used a hammerhead ribozyme to down-regulate Fas-L in human glioma cells and observed the effects in vitro and in nude mice.
Digital Object Identifier (DOI)
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