Isolation and Identification of Aspergillus Tubingensis for Deoxynivalenol Biotransformation
Author Information
Author(s): He Chenghua, Fan Yanhong, Liu Guofang, Zhang Haibin
Primary Institution: Nanjing Agricultural University
Hypothesis
Can a strain of Aspergillus tubingensis effectively biotransform deoxynivalenol (DON)?
Conclusion
The strain NJA-1 of Aspergillus tubingensis can biotransform deoxynivalenol with a mean transformation rate of 94.4%.
Supporting Evidence
- Only one strain was isolated from fifteen soil samples and five rumen content samples.
- The mean DON biotransformation rate was 94.4% after two weeks of cultivation.
- The DNA sequence of the PCR product had 99% similarity to Aspergillus tubingensis.
- The biotransformation product had a molecular weight 18.1 D (H2O) larger than that of DON.
Takeaway
Scientists found a type of fungus that can change a harmful substance in food into something less toxic.
Methodology
The study involved isolating a strain of Aspergillus tubingensis from soil and testing its ability to biotransform deoxynivalenol using HPLC analysis.
Limitations
Further studies are needed to identify the biotransformation product and compare its cytotoxicity with that of DON.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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