Development of a Rapid Test for Foot-and-Mouth Disease
Author Information
Author(s): Wu Lei, Jiang Tao, Lu Zeng-Jun, Yang Ya-Min, Sun Pu, Liang Zhong, Li Dong, Fu Yuan-Fang, Cao Yi-Mei, Liu Xiang-Tao, Liu Zai-Xin
Primary Institution: State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Hypothesis
Can a new immunochromatographic strip effectively differentiate between infected and vaccinated animals for foot-and-mouth disease?
Conclusion
The immunochromatographic strip is a reliable tool for quickly diagnosing foot-and-mouth disease in animals.
Supporting Evidence
- The coincidence rate for pigs was 100% for negative serum, 97.2% for vaccinated serum, and 95.0% for infected serum.
- The coincidence rate for cattle was 100% for negative serum, 96.7% for vaccinated serum, and 98.0% for infected serum.
- The coincidence rate for sheep was 97.6% for negative serum and 96.3% for infected serum.
Takeaway
Scientists created a simple test that helps tell if animals are sick with foot-and-mouth disease or just vaccinated.
Methodology
The study involved developing an immunochromatographic strip using 2C'3AB protein and testing it against 387 serum samples.
Limitations
The test's sensitivity may decrease over time at higher temperatures.
Participant Demographics
The study included serum samples from cattle, pigs, and sheep.
Digital Object Identifier (DOI)
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