Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system
2011
New Expression System for Lactococcus lactis
publication
Evidence: moderate
Author Information
Author(s): Douillard François P, O'Connell-Motherway Mary, Cambillau Christian, van Sinderen Douwe
Primary Institution: University College Cork
Hypothesis
Can a thioredoxin gene fusion expression system improve protein production in Lactococcus lactis?
Conclusion
The thioredoxin gene fusion expression system allows for the efficient production of soluble proteins in Lactococcus lactis.
Supporting Evidence
- The thioredoxin fusion system was shown to prevent protein insolubility and degradation.
- High-level expression of soluble heterologous proteins was achieved.
- Purification of proteins was successfully performed using Ni-TED affinity chromatography.
Takeaway
This study created a new way to help bacteria make proteins better, so they don't get all tangled up and messy.
Methodology
Two nisin-inducible expression vectors were constructed to over-produce proteins in Lactococcus lactis as thioredoxin fusion proteins.
Limitations
The study does not demonstrate the functionality of the overexpressed proteins.
Digital Object Identifier (DOI)
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