HuR binding to AU-rich elements present in the 3' untranslated region of Classical swine fever virus
2011
HuR Binding to the 3' UTR of Classical Swine Fever Virus
publication
Evidence: moderate
Author Information
Author(s): Nadar Muthukumar, Chan Meng-Yu, Huang Shi-Wei, Huang Chin-Cheng, Tseng Joseph T, Tsai Ching-Hsiu
Primary Institution: Graduate Institute of Biotechnology, National Chung Hsing University
Hypothesis
This study inspects a possible role of the ARE in the 3' UTR of CSFV.
Conclusion
The study identified that HuR specifically binds to the ARE region in the 3' UTR of CSFV.
Supporting Evidence
- HuR is known to bind the AREs and protect the mRNA from degradation.
- HuR binding ability is conserved in strains irrespective of virulence.
- CSFV harbors bona fide AREs in the 3' UTR of its genomic RNA.
Takeaway
The study found that a protein called HuR sticks to a part of the virus's genetic material, which helps the virus survive longer.
Methodology
The study used RNA pull-down and LC/MS/MS assays to identify host factors binding to the CSFV 3' UTR.
Digital Object Identifier (DOI)
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