Conformational alterations in the CD4 binding cavity of HIV-1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV-1 envelopes derived from brain and other tissues
2011

HIV-1 Envelope Changes Affect CD4 Binding and Virus Fusion

Sample size: 81 publication Evidence: moderate

Author Information

Author(s): Lachlan Gray, Jasminka Sterjovski, Paul A Ramsland, Melissa J Churchill, Paul R Gorry

Primary Institution: Burnet Institute

Hypothesis

How do conformational changes in the CD4 binding site of HIV-1 gp120 influence its interactions with CD4 and fusogenicity?

Conclusion

The study confirms that structural changes in the CD4 binding site of gp120 enhance its ability to interact with CD4 and increase fusogenicity.

Supporting Evidence

  • The study utilized a larger panel of Envs derived from brain and other tissues to validate previous findings.
  • Increased aperture width of the CD4 binding site was associated with enhanced fusogenicity.
  • The study confirmed that structural alterations in the CD4 binding site can influence Env function.

Takeaway

The study shows that changes in a part of the HIV virus help it stick better to cells, which can make it more harmful.

Methodology

Three-dimensional models of gp120 were created and analyzed for their CD4 binding site characteristics and fusogenicity.

Participant Demographics

Envs derived from brain and other tissues of 7 subjects who died from AIDS.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1742-4690-8-42

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