Mapping the Genome of Mycobacterium avium subspecies paratuberculosis
Author Information
Author(s): Wu Chia-wei, Schramm Timothy M, Zhou Shiguo, Schwartz David C, Talaat Adel M
Primary Institution: University of Wisconsin-Madison
Hypothesis
Can optical mapping reveal genomic variations between strains of Mycobacterium avium subspecies paratuberculosis?
Conclusion
The study confirmed that the sequenced genome of M. ap K-10 has assembly errors and identified two new genes in the M. ap K-10 genome.
Supporting Evidence
- The optical map of M. ap ATCC 19698 showed a genome size of 4,839 kb.
- A 648-kb inversion was identified around the origin of replication.
- Two new copies of coding sequences were found in the M. ap K-10 genome.
Takeaway
Scientists looked at the DNA of two types of bacteria to see how they are different. They found some mistakes in the DNA of one type and discovered new parts of its DNA.
Methodology
Optical mapping was used to profile genomic variations between strains of M. ap K-10 and M. ap ATCC 19698.
Limitations
The resolution of optical mapping is lower compared to whole-genome sequencing, which may miss some smaller variations.
Digital Object Identifier (DOI)
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