Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol
Author Information
Author(s): Leelawat Kawin, Narong Siriluck, Chaijan Suthidarak, Sa-ngiamsuntorn Khanit, Disthabanchong Sinee, Wongkajornsilp Adisak, Hongeng Suradej
Primary Institution: Department of Surgery, Rajavithi Hospital, Rajathevi, Bangkok, Thailand
Hypothesis
The study investigates the effects of a liver differentiation protocol on the proteomic profiles of mesenchymal stem cells (MSCs).
Conclusion
The study found that MSCs treated with a liver differentiation protocol expressed significantly more liver-specific proteins compared to undifferentiated cells.
Supporting Evidence
- MSCs treated with the liver differentiation protocol expressed significantly more albumin, CK19, and CK20 than undifferentiated cells.
- Increased expression of FEM1B, PSMC2, and disulfide-isomerase A3 was found in MSCs treated with the liver differentiation protocol.
- The study demonstrated that differentiated MSCs maintained viability and characteristic hepatic phenotypic markers over time.
Takeaway
Scientists looked at how special cells from bone marrow can turn into liver-like cells, and they found that these cells start to act more like liver cells when treated with a specific method.
Methodology
The study used Western blot analyses, two-dimensional gel electrophoresis, and RT-PCR to assess protein expression in MSCs treated with a liver differentiation protocol.
Participant Demographics
Cells were derived from six donors of both sexes.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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