Cyclic changes in gene expression induced by Peg-interferon alfa-2b plus ribavirin in peripheral blood monocytes (PBMC) of hepatitis C patients during the first 10 weeks of treatment
2008

Gene Expression Changes in Hepatitis C Patients Treated with Peg-Interferon and Ribavirin

Sample size: 20 publication Evidence: moderate

Author Information

Author(s): Taylor Milton W, Tsukahara Takuma, McClintick Jeanette N, Edenberg Howard J, Kwo Paul

Primary Institution: Indiana University

Hypothesis

This study aims to determine the kinetics of gene expression during the first 10 weeks of therapy with Pegylated-interferon-alfa2b and ribavirin in HCV patients.

Conclusion

The response to PegIntron/ribavirin was similar to that reported for Pegasys/ribavirin, suggesting that more frequent dosing might improve treatment response.

Supporting Evidence

  • Gene expression levels were significantly altered at various time points during treatment.
  • 11 out of 20 patients showed a positive response to therapy by week 10.
  • The sustained viral response rate was 35% at the end of treatment.

Takeaway

This study looked at how certain genes change when patients with hepatitis C are treated with a specific medicine. It found that these changes happen in a pattern and that giving the medicine more often might help patients more.

Methodology

RNA was isolated from PBMC of treatment-naïve patients at multiple time points during treatment, and gene expression was measured using Affymetrix microarrays.

Limitations

The study had limited power to detect differences between responders and non-responders due to the small sample size.

Participant Demographics

16 males and 4 females, all genotype 1 hepatitis C patients.

Statistical Information

P-Value

p ≤ 0.001

Statistical Significance

p ≤ 0.001

Digital Object Identifier (DOI)

10.1186/1479-5876-6-66

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