ATF3 as an Oncogene in Mouse Mammary Tumorigenesis
Author Information
Author(s): Wang Aijin, Arantes Stacey, Yan Leqin, Kiguchi Kaoru, McArthur Mark J, Sahin Aysegul, Thames Howard D, Aldaz C Marcelo, MacLeod Michael C
Primary Institution: The University of Texas M.D. Anderson Cancer Center
Hypothesis
Does overexpression of ATF3 in CK5-expressing cells lead to mammary tumorigenesis in mice?
Conclusion
Overexpression of ATF3 in CK5-expressing cells leads to squamous metaplastic lesions and mammary tumors in mice, indicating its role as an oncogene.
Supporting Evidence
- Transgenic BK5.ATF3 mice developed squamous metaplastic lesions by 25 weeks of age.
- Biparous BK5.ATF3 mice had a 67% incidence of mammary carcinomas by one year of age.
- Human breast tumors showed high levels of nuclear ATF3 expression.
Takeaway
When a specific gene called ATF3 is overproduced in certain cells of mice, it can cause abnormal growths in their breasts, similar to tumors in humans.
Methodology
Mammary glands from transgenic and non-transgenic mice were examined for anomalies using histopathology and immunohistochemistry.
Potential Biases
Potential bias in the selection of mouse lines and the interpretation of histopathological results.
Limitations
The study primarily focuses on a specific mouse model, which may not fully represent human breast cancer.
Participant Demographics
Transgenic and non-transgenic female mice, including nulliparous and biparous.
Statistical Information
P-Value
0.0005
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website