Engineering Priestia megaterium to Produce 2'-Fucosyllactose
Author Information
Author(s): Park Bu-Soo, Yoon Jihee, Lee Jun-Min, Cho Sang-Hyeok, Choi Yoojeong, Cho Byung-Kwan, Oh Min-Kyu
Primary Institution: Korea University
Hypothesis
Can the GRAS strain Priestia megaterium be engineered to produce 2'-fucosyllactose efficiently?
Conclusion
The engineered Priestia megaterium successfully produced 28.6 g/L of 2'-fucosyllactose through genetic modifications.
Supporting Evidence
- The engineered strain achieved a 2'-FL production level of 28.6 g/L in fed-batch fermentation.
- Genetic modifications included deletion of the lacZ gene and overexpression of the futC gene.
- Random mutagenesis was employed to enhance the strain's productivity.
Takeaway
Scientists changed a safe bacteria to help it make a special sugar found in breast milk, which is good for babies.
Methodology
The study involved genetic modifications of Priestia megaterium, including gene deletions and overexpressions to enhance 2'-fucosyllactose production.
Limitations
The production levels achieved have not yet reached those of other strains, indicating room for further optimization.
Digital Object Identifier (DOI)
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