Paraoxonase 1 (PON1) Polymorphisms, Haplotypes and Activity in Predicting CAD Risk in North-West Indian Punjabis
2011

PON1 Polymorphisms and CAD Risk in North-West Indian Punjabis

Sample size: 650 publication 10 minutes Evidence: high

Author Information

Author(s): Nidhi Gupta, Surjit Singh, Maturu V. Nagarjuna, Yash Paul Sharma, Kiran Dip Gill

Primary Institution: Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India

Hypothesis

The polymorphisms of the PON1 gene affect PON1 activity and thereby coronary artery disease (CAD) risk in North-West Indian Punjabis.

Conclusion

CAD patients had lower PONase and AREase activities compared to controls, and specific PON1 polymorphisms and haplotypes are independently associated with CAD.

Supporting Evidence

  • The study found that CAD patients had significantly lower PONase and AREase activities compared to controls.
  • Specific PON1 polymorphisms were associated with increased CAD risk.
  • Haplotypes L-T-G-Q-C and L-T-G-R-G were significantly associated with higher CAD risk.

Takeaway

This study found that people with heart disease had lower levels of a protective enzyme, and certain gene variations can increase the risk of heart disease.

Methodology

The study involved 350 CAD patients and 300 healthy controls, measuring PON1 activity and genotyping using PCR methods.

Potential Biases

Potential bias due to the inability to control for statin effects on PON1 activity.

Limitations

The study could not stop statin treatment in CAD patients, which may influence PON1 activity.

Participant Demographics

Participants included North-West Indian Punjabis, with 350 CAD patients and 300 healthy controls.

Statistical Information

P-Value

0.0001

Confidence Interval

1.57–4.72 for QR genotype; 6.41–41.14 for RR genotype

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0017805

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