The parafibromin tumor suppressor protein interacts with actin-binding proteins actinin-2 and actinin-3
2008
Parafibromin and Its Interaction with Actinins
Sample size: 106
publication
Evidence: moderate
Author Information
Author(s): Sunita K Agarwal, William F Simonds, Stephen J Marx
Primary Institution: National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland, USA
Hypothesis
The study aims to identify proteins that interact with the parafibromin tumor suppressor protein.
Conclusion
Parafibromin interacts with muscle actinins and may play a role in actin bundling and cross-linking.
Supporting Evidence
- Parafibromin was found to interact with muscle alpha-actinins actinin-2 and actinin-3.
- The interaction was verified through yeast two-hybrid, GST pull-down, and co-immunoprecipitation methods.
- Parafibromin was predominantly nuclear in undifferentiated myoblasts but co-localized with actinins in differentiated myotubes.
Takeaway
Parafibromin, a protein that helps prevent tumors, works with other proteins called actinins to help organize muscle cells.
Methodology
Yeast two-hybrid system was used to screen a heart cDNA library for parafibromin interacting proteins.
Digital Object Identifier (DOI)
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