Comprehensive Classification of Methyltransferases in Yeast
Author Information
Author(s): Wlodarski Tomasz, Kutner Jan, Towpik Joanna, Knizewski Lukasz, Rychlewski Leszek, Kudlicki Andrzej, Rowicka Maga, Dziembowski Andrzej, Ginalski Krzysztof
Primary Institution: University of Warsaw
Hypothesis
The study aims to provide a detailed classification of the methyltransferases encoded in the Saccharomyces cerevisiae genome.
Conclusion
The study identifies 86 methyltransferases in S. cerevisiae, including 34 RNA MTases, 32 protein MTases, eight small molecule MTases, and three lipid MTases.
Supporting Evidence
- The study identified 86 methyltransferases in S. cerevisiae.
- 34 of these are RNA MTases, while 32 are protein MTases.
- New methods were developed to predict substrate specificity based on gene expression data.
- Experimental validation confirmed the predicted substrate specificity for two MTases.
Takeaway
Scientists studied yeast to find out how many different enzymes help add methyl groups to molecules, and they found 86 of them!
Methodology
The study used distant homology detection and fold recognition to classify methyltransferases based on their structure and substrate specificity.
Potential Biases
Potential bias in the identification of putative MTases due to reliance on computational predictions.
Limitations
The study may not cover all potential methyltransferases due to reliance on existing databases and bioinformatic predictions.
Statistical Information
P-Value
7×10−6
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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