Analysis of the transcriptional activity of endogenous NFAT5 in primary cells using transgenic NFAT-luciferase reporter mice
2008
Study of NFAT5 Activation in Cells
publication
Evidence: moderate
Author Information
Author(s): Morancho Beatriz, Minguillón Jordi, Molkentin Jeffery D, López-Rodríguez Cristina, Aramburu Jose
Primary Institution: Universitat Pompeu Fabra
Hypothesis
The study investigates the transcriptional activity of NFAT5 in response to hypertonicity in primary cells.
Conclusion
NFAT5 is sensitive to increases in extracellular tonicity in T lymphocytes, with activation thresholds varying by cell type.
Supporting Evidence
- The NFAT5 transcription factor is activated by hypertonicity in T lymphocytes.
- Activation thresholds for NFAT5 vary among different cell types.
- The study utilized a transgenic mouse model to analyze NFAT5 activity.
Takeaway
This study shows that a protein called NFAT5 helps cells respond to high salt levels, especially in immune cells like T lymphocytes.
Methodology
The study used transgenic mice with a luciferase reporter to measure NFAT5 activation in response to hypertonicity in various primary cell types.
Digital Object Identifier (DOI)
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