Creating Antibodies to Reduce CD147 in Cells
Author Information
Author(s): Tragoolpua Khajornsak, Intasai Nutjeera, Kasinrerk Watchara, Mai Sabine, Yuan Yuan, Tayapiwatana Chatchai
Primary Institution: Chiang Mai University
Hypothesis
Can an intrabody be generated to down-regulate the expression of CD147 on 293A cells?
Conclusion
The study successfully demonstrates that an intrabody can effectively reduce the expression of CD147 on the surface of 293A cells.
Supporting Evidence
- The scFv-M6-1B9 intrabody was successfully expressed in 293A cells.
- CD147 expression on the cell surface was significantly reduced after transduction with the intrabody.
- Colocalization of the intrabody with CD147 was observed in the endoplasmic reticulum.
Takeaway
Scientists made a special type of antibody that can stop a protein called CD147 from being on the outside of certain cells, which could help us understand how this protein works.
Methodology
The study used phage display technology to create a CD147-specific scFv intrabody, which was then expressed in 293A cells via adenoviral gene transfer.
Limitations
The study does not address the long-term effects of CD147 down-regulation or the potential off-target effects of the intrabody.
Digital Object Identifier (DOI)
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