Using Antibodies to Find Tumors
Author Information
Author(s): A. Jeppsson, B. Wahren, J.L. Millan, T. Stigbrand
Primary Institution: Department of Physiological Chemistry, University of Umea; Department of Virology, National Bacteriological Laboratory
Hypothesis
Can placental alkaline phosphatase (PLAP) be used as a marker for tumor localization in humans?
Conclusion
The study found that both monoclonal and polyclonal antibodies against PLAP effectively localized tumors in nude mice.
Supporting Evidence
- Antibodies against PLAP localized effectively to Hep 2 tumors in nude mice.
- Concentration ratios of antibodies in tumors were significantly higher than in other tissues.
- 100% of Hep 2 cells in culture showed positivity for PLAP.
Takeaway
Researchers used special antibodies to find tumors in mice, and they discovered that these antibodies worked really well for tumors that produced a specific protein.
Methodology
The study involved immunizing rabbits to produce antibodies against PLAP, which were then used to localize tumors in nude mice.
Limitations
The study was conducted in mice, which may not fully represent human responses.
Participant Demographics
Nude mice were used as the animal model.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
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